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From one portion, free IAA was determined as described above. Total IAA was measured in the other portion by subjecting the extract to hydrolysis in 7 n NaOH at 100°C for 3 h in a N2 atmosphere, neutralizing with HCl, and quantifying as… Immunocytochemistry for tubulin, rabies virus antigen, p50 subunit of NF-κB, and 4-hydroxy-2-nonenal adducts.CVS- and mock-infected cultured DRG neurons at 72 h postinfection (p.i.) were fixed with 4% paraformaldehyde in phosphate-buffered… However, the precise mechanisms responsible for the dysregulation of adiponectin have not been fully elucidated. After blocking with 3% normal horse serum (NHS) in 0.01% Triton X-100, cells were incubated with anti-mouse BrdU (1:1000; Boehringer-Mannheim, Indianapolis, IN) overnight at 4°C. Sections were then incubated for 1 hr with secondary antibody… Immunohistochemistry was then performed according to the instructions of the manufacturer (Vector Laboratories). Our protocol includes deparaffination, rehydration in alcohol up to water, antigen retrieval in citrate buffer, pH 6.0… Michael Jackson: The Immortal World Tour was the first of two theatrical productions by Cirque du Soleil to combine the music of Michael Jackson with Cirque du Soleil's signature acrobatic performance style. Supporting their eighth studio album, In a World Like This, the tour consisted of over 150 shows in Asia, North America and Europe.
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The Wall Live was a worldwide concert tour by Roger Waters, formerly of Pink Floyd. The tour is the first time the Pink Floyd album The Wall has been performed in its entirety by the band or any of its former members since Waters performed… This approach led to success in cell-free cloning of individual DNA molecules by using submicroliter reaction volumes. Finally, the membrane was washed three times with TBS-T and developed using the ECL procedure according to the manufacturer's instructions. Bacterial strains and plasmids.Strains and plasmids used in this study are listed in Table 1. Restriction enzymes, phosphatase (New England BioLabs, Ipswich, MA), ligase (rapid DNA ligation kit; Roche, Mannheim, Germany), and KOD DNA… The gene expression libraries were prepared using the Illumina Gene Expression Sample Preparation Kit (Illumina San Diego, CA) according to the manufacturer's instructions. We like to thank D. Ballert; T. Sonntag, E. Xie for experimental help, B. Lepetit for helpful discussions, the FlowKon Core Facility, Universität Konstanz, for access to the flow cytometer FACSCalibur and S. DNA probes were labeled with α-32P-dCTP (Amersham Pharmacia Biotech, Buckinghamshire, UK) with the Bcabest labeling kit (Takara, Ohtsu, Japan) or labeled with digoxigenin-dUTP (Boehringer Mannheim) with the Bcabest digoxigenin labeling kit…
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DNA probes were labeled with α-32P-dCTP (Amersham Pharmacia Biotech, Buckinghamshire, UK) with the Bcabest labeling kit (Takara, Ohtsu, Japan) or labeled with digoxigenin-dUTP (Boehringer Mannheim) with the Bcabest digoxigenin labeling kit… Song W, Kong HL, Carpenter H, et al Dendritic cells genetically modified with an adenovirus vector encoding the cDNA for a model antigen induce protective and therapeutic antitumor immunity. Purification of DNA from infected cells and CsCl purified virions was performed by overnight digestion at 37°C in pronase-SDS buffer (10 mM Tris·HCl, pH 7.4/10 mM EDTA/1% SDS/1 mg/ml pronase), phenol extraction, and ethanol precipitation. The hybridized oligonucleotide probe was detected by using the DIG DNA Labeling and Detection Kit with the chemiluminescent substrate CSPD (Boehringer Mannheim) and exposure to x-ray film, or with the fluorimetric substrate AttoPhos… The As I Am Tour was the third worldwide concert tour by American singer/songwriter Alicia Keys, in support of her third studio album, As I Am. It continued through the Americas, Asia, Oceania and Europe until February 5, 2016, in Amsterdam, Netherlands. The band played 108 shows. Three CD49d-transfected clones (clones 16, 29, and 39) were tested for autoreactive proliferation response against irradiated syngeneic splenocytes.
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